Day 43 ( Second Lab Session )
Haha.. today thurs 2nd Oct 2008, i had my second lab session!
Eh.. using the scarpel to cut off the hypocotyl..
Dipping the scarpel into high concentration of A. Tumefacian, we tried to cut a few times at the target region to introduce the bacteria into the seed. Next we sonicate the solution:
and vacuum the solution.. haha.. they say the sonication and the vacuum actually increase the efficiency of DNA uptake into the wounded seedlings~
Putting the seedling into our culture medium.. eh.. pai say ar.. we don't have the culture to wear gloves here.. LOL!!
Haha.. today is just another schooling day in Dong-A University. But tomorrow will be more exciting! A trip to Jinju for the lantern festival! Do look out for the next post!
Eh.. using the scarpel to cut off the hypocotyl..
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